In Vitro Propagation of Aconitum violaceum Jacq. ex Stapf through Seed Culture and Somatic Embryogenesis

Aconitum violaceum Jacq. ex Stapf is a threatened medicinal plant with restricted global distribution. The highest frequency of seed germination was recorded on Murashige and Skoog’s (MS) basal medium, supplemented 0.5 mg L?1 kinetin rate 77.32% mean time 27 days. Among the various growth regulators examined, 0.1 (Kn) + indole-3-acetic acid (IAA) proved to be effective for maximum embryogenic callus production (51.0%) within 31 days inoculation. conversion somatic embryos into complete plantlets in MS medium augmented Kn IAA (68.00%), an average root initiation 25 rooted were subsequently hardened jiffy pots combination loamy soil, coco-peat, vermicompost (1:1:1 v/v), then transplanted greenhouse 60% survival rate. To our knowledge, this first study direct vitro propagation induction from seeds. established regeneration protocol could employed propagate A. large scale short time. This would contribute significantly its rapid germplasm conservation, establish framework domestication highly valued plant.